# Molecular Biology Techniques and Applications

**Type:** Topics  
**Canonical URL:** https://scholariq.org/topics/molecular-biology-techniques-and-applications/

## Facts

| Field | Value |
| --- | --- |
| Description | This cluster of papers focuses on the use of real-time polymerase chain reaction (PCR) for quantitative analysis of gene expression, including topics such as normalization strategies, reference gene selection, RNA integrity assessment, and data analysis methods. The MIQE guidelines for minimum information in publication of quantitative real-time PCR experiments are emphasized. |
| Domain | Life Sciences |
| Field | Biochemistry, Genetics and Molecular Biology |
| OpenAlex ID | t11970 |
| Works | 146 |

## Topic papers all

Showing 15 of 146.

- [Robust enumeration of cell subsets from tissue expression profiles](https://scholariq.org/papers/robust-enumeration-of-cell-subsets-from-tissue-expression-profiles/)
- [Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications](https://scholariq.org/papers/gene-expression-patterns-of-breast-carcinomas-distinguish-tumor-subclasses-with/)
- [Significance analysis of microarrays applied to the ionizing radiation response](https://scholariq.org/papers/significance-analysis-of-microarrays-applied-to-the-ionizing-radiation-response/)
- [Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling](https://scholariq.org/papers/distinct-types-of-diffuse-large-b-cell-lymphoma-identified-by-gene-expression/)
- [Proposed minimum reporting standards for chemical analysis](https://scholariq.org/papers/proposed-minimum-reporting-standards-for-chemical-analysis/)
- [Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ](https://scholariq.org/papers/screening-gt-recombinant-clones-by-hybridization-to-single-plaques-in-situ/)
- [Development and validation of a clinical cancer genomic profiling test based on massively parallel DNA sequencing](https://scholariq.org/papers/development-and-validation-of-a-clinical-cancer-genomic-profiling-test-based-on/)
- [Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.](https://scholariq.org/papers/method-for-analysis-of-cellular-dna-content-of-paraffin-embedded-pathological/)
- [Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA](https://scholariq.org/papers/design-and-evaluation-of-useful-bacterium-specific-pcr-primers-that-amplify/)
- [Noninvasive Identification and Monitoring of Cancer Mutations by Targeted Deep Sequencing of Plasma DNA](https://scholariq.org/papers/noninvasive-identification-and-monitoring-of-cancer-mutations-by-targeted-deep/)
- [Exploring the phenotypic consequences of tissue specific gene expression variation inferred from GWAS summary statistics](https://scholariq.org/papers/exploring-the-phenotypic-consequences-of-tissue-specific-gene-expression/)
- [Integrated Proteogenomic Characterization of Human High-Grade Serous Ovarian Cancer](https://scholariq.org/papers/integrated-proteogenomic-characterization-of-human-high-grade-serous-ovarian/)
- [RETRACTED ARTICLE: Microbiome analyses of blood and tissues suggest cancerdiagnostic approach](https://scholariq.org/papers/retracted-article-microbiome-analyses-of-blood-and-tissues-suggest/)
- [Molecular Classification and Molecular Forecasting of Breast Cancer: Ready for Clinical Application?](https://scholariq.org/papers/molecular-classification-and-molecular-forecasting-of-breast-cancer-ready-for/)
- [The MicroArray Quality Control (MAQC)-II study of common practices for the development and validation of microarray-based predictive models](https://scholariq.org/papers/the-microarray-quality-control-maqc-ii-study-of-common-practices-for-the/)

## Topic primary papers

Showing 15 of 41.

- [Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ](https://scholariq.org/papers/screening-gt-recombinant-clones-by-hybridization-to-single-plaques-in-situ/)
- [Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA](https://scholariq.org/papers/design-and-evaluation-of-useful-bacterium-specific-pcr-primers-that-amplify/)
- [Measurement of Gene Expression in Archival Paraffin-Embedded Tissues](https://scholariq.org/papers/measurement-of-gene-expression-in-archival-paraffin-embedded-tissues/)
- [Application of comparative functional genomics to identify best-fit mouse models to study human cancer](https://scholariq.org/papers/application-of-comparative-functional-genomics-to-identify-best-fit-mouse-models/)
- [Use of a monoclonal anti-estrogen receptor antibody in the immunohistochemical evaluation of human tumors.](https://scholariq.org/papers/use-of-a-monoclonal-anti-estrogen-receptor-antibody-in-the-immunohistochemical/)
- [Solid-phase reversible immobilization for the isolation of PCR products](https://scholariq.org/papers/solid-phase-reversible-immobilization-for-the-isolation-of-pcr-products/)
- [A Cellular Anatomy of the Normal Adult Human Prostate and Prostatic Urethra](https://scholariq.org/papers/a-cellular-anatomy-of-the-normal-adult-human-prostate-and-prostatic-urethra/)
- [GWAS meta-analysis and replication identifies three new susceptibility loci for ovarian cancer](https://scholariq.org/papers/gwas-meta-analysis-and-replication-identifies-three-new-susceptibility-loci-for/)
- [Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida](https://scholariq.org/papers/validation-of-reference-genes-for-quantitative-real-time-pcr-during-leaf-and/)
- [Double stranded DNA sequencing as a choice for DNA sequencing](https://scholariq.org/papers/double-stranded-dna-sequencing-as-a-choice-for-dna-sequencing/)
- [FastPCR Software for PCR Primer and Probe Design and Repeat Search](https://scholariq.org/papers/fastpcr-software-for-pcr-primer-and-probe-design-and-repeat-search/)
- [Correction: Corrigendum: Global quantification of mammalian gene expression control](https://scholariq.org/papers/correction-corrigendum-global-quantification-of-mammalian-gene-expression/)
- [Presence of Filterable and Nonfilterable mRNA in the Plasma of Cancer Patients and Healthy Individuals](https://scholariq.org/papers/presence-of-filterable-and-nonfilterable-mrna-in-the-plasma-of-cancer-patients/)
- [Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis](https://scholariq.org/papers/oral-cancer-in-vivo-gene-expression-profiling-assisted-by-laser-capture/)
- [Independent Component Analysis Uncovers the Landscape of the Bladder Tumor Transcriptome and Reveals Insights into Luminal and Basal Subtypes](https://scholariq.org/papers/independent-component-analysis-uncovers-the-landscape-of-the-bladder-tumor/)

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Source: ScholarIQ — public research metadata, principally OpenAlex. See https://scholariq.org/sources/ for provenance and https://scholariq.org/methodology/ for what these figures mean.