Peuplements végétaux et bioagresseurs en milieu tropical

Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) are chemogenetic tools for remote control of targeted cell populations using chemical actuators that bind to modified receptors. Despite the popularity of DREADDs in neuroscience and sleep research, potential effects of clozapine-N-oxide (CNO) and other DREADD actuators on sleep have never been systematically tested. Recent reports suggest that CNO can affect brain activity and animal behaviour, putatively through conversion to clozapine. The aim of our study was to assess whether CNO or compound 21 (C21), a novel actuator that cannot convert to clozapine, affect sleep in wild type laboratory mice.